Water Science and Engineering 2018, 11(1) 17-22 DOI:   https://doi.org/10.1016/j.wse.2018.03.006  ISSN: 1674-2370 CN: 32-1785/TV

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Keywords
Denitrifying phosphorus removal
pH
Removal rate
PHB
Authors
PubMed

Influence of pH on short-cut denitrifying phosphorus removal

Wei Li a,*, Hui-yan Zhang a, Hui-zhi Sun a, Fei Zeng a, Yu-nan Gao a, Lei Zhu b

a School of Municipal and Environmental Engineering, Shenyang Jianzhu University, Shenyang 110168, China b Shenyang Academy of Environmental Sciences, Shenyang 110167, China

Abstract

Through a series of experiments using denitrifying phosphorus-accumulating sludge in sequencing batch reactors (SBRs), the variations of the intracellular polymers during the anaerobic phosphorus release process at different pH values were compared, the probable reasons for different performances of phosphorus removal were examined, and system operations in a typical cycle were investigated. The results show that the phosphorus removal rate was positively correlated with pH values in a range of 6.5 to 8.5. When the pH value was 8.0, the anaerobic phosphorus release rate and anoxic phosphorus uptake rate of the activated sludge were 20.95 mg/(g?h) and 23.29 mg/(g?h), respectively; the mass fraction of poly-β-hydroxybutyrate (PHB) increased to 62.87 mg/g under anaerobic conditions; the mass fraction of polyphosphate was 92.67 mg/g under anoxic conditions; and the effluent concentration of TP was 1.47 mg/L. With the increase of pH, the mass fraction of acetic acid and PHB also increased, and the absorption rate of acetic acid was equal to the disintegration rate of polyphosphate. When the pH value was above 8.0, biological phosphorus removal was achieved by chemical phosphorus precipitation, and the phosphorus removal rate decreased.

Keywords Denitrifying phosphorus removal   pH   Removal rate   PHB  
Received 2017-03-03 Revised 2017-10-30 Online: 2018-01-31 
DOI: https://doi.org/10.1016/j.wse.2018.03.006
Fund:

This work was supported by the Research Program of the Liaoning Educational Committee (Grant No. LJZ2016014), the Natural Science Foundation of Liaoning Province (Grant No. 201501069), the Research Program of the Ministry of Housing and Urban-Rural Development (Grant No. 2015-K7-007), and the National Natural Science Foundation of China (Grants No. 51508342 and 51476107).

Corresponding Authors: liweilengjinyue@163.com (Wei Li)
Email: liweilengjinyue@163.com
About author: liweilengjinyue@163.com (Wei Li)

References:

Chua, A.S.M., Takabatake, H., Satoh, H., Mino, T., 2013. Production of polyhydroxyalkanoates (PHA) by activated sludge treating municipal wastewater: Effect of pH, sludge retention time (SRT), and acetate concentration in influent. Water Research, 37(15), 3602–3611. https://doi.org/10.1016/s0043-1354(03)00252-5.

Deng, Q.Q., 2011. Technique of short-cut denitrifying dephosphorization and its study progress. Guangdong Chemical Industry, 3, 34–35 (in Chinese).

Ding, W.C., Li, Q., Ji, F.Y., Tian, X.M., Wu, D., Du, Y., 2010. Shortcut nitrification-denitrification and denitrifying phosphorus removal in two-sludge SBR system. China Water and Wastewater, 26(13), 11–14 (in Chinese).

Fleit, E., 1995. Intracellular pH regulation in biological excess phosphorous removal systems. Water Research, 29(7), 87–92. https://doi.org/10.1016/0043-1354(94)00265-9.

Guo, H.J., Ma, F., Shen, Y.L., 2005a. Effect of C/N ration on denitrifying dephosphatation. Acta Scientiae Circumstantiae, 25(3), 367–371. https://doi.org/10.3321/j.issn:0253-2468.2005.03.016.

Guo, X., Meng, Z.H., Dong, J.H., 2005b. Study on pH value effect on removal of biological phosphorus in anaerobic pool. Journal of Harbin University of Commerce (Sciences Edition), 21(3), 292–293. https://doi.org/10.19492/j.cnki.1672-0946.2005.03.009.

Jabari, P., Munz, G., Oleszkiewicz, J.A., 2014. Selection of denitrifying phosphorous accumulating organisms in IFAS systems: Comparison of nitrite with nitrate as an electron acceptor. Chemosphere, 109, 20–27. https://doi.org/10.1016/j.chemosphere.2014.03.002.

Li, J., Chai, L.Y., Xiang, R.J., Cheng, Y.X., 2011. Pilot-scale test on domestic waste water treatment using integrative A/O (anaerobic-aerobic sludge) equipment. Journal of Central South University: Science and Technology, 42(10), 2935–2940 (in Chinese).

Li, N., 2010. Performance and Removal Approaches of Biological Phosphorus Removal from Wastewater in SBR under Low Temperature. Ph. D. Dissertation. Harbin University of Technology, Harbin (in Chinese).

Li, X.K., 2006. Study on Denitrifying Phosphorus Removal Process and Microbiology Research. Ph. D. Dissertation. Harbin Institute of Technology, Harbin (in Chinese).

Li, X.K., Zhang, J., Huang, R.X., Ma, L., Bao. R.L., Jiang, A.X., 2006. Study on characteristic of denitrification phosphorus removal bacteria. China Water and Wastewater, 22(3), 35–39 (in Chinese).

Nittami, T., Oi, H., Matsumoto, K., Seviour, R.J., 2010. Influence of temperature, pH and dissolved oxygen concentration on enhanced biological phosphorus removal under strictly aerobic conditions. Journal of Biotechnology, 150, 237. https://doi.org/10.1016/j.jbiotec.2010.09.090.

Peng, Y.Z., 2011. SBR method for sewage biological phosphorus removal and process control. Science Press, 188–190 (in Chinese).

Smolders, G.J.F., van der Meij, J., van Loosdrecht, M.C.M., Heijnen, J.J., 1994. Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence. Biotechnology and Bioengineering, 43(6), 461–470. https://doi.org/10.1002/bit.260430605.

Wang, A.J., Wu, L.H., Ren, N.Q., Zhao, D., Yan, M.L., 2005. Feasibility of denitrifying phosphorus removal technique using nitrite as electron acceptor. China Environmental Science, 25(5), 515–518 (in Chinese).

Wang, J.H., Peng, Y.Z., Chen, Y.Z., 2011a. Experimental research on suspended aerobic biofilm A2O system for treating domestic wastewater. Journal of Central South University: Science and Technology, 42(12), 3918–3922 (in Chinese).

Wang, Y.Y., Geng, J.J., Ren, Z.J., He, W.T., Xing, M.Y., Wu, M., Chen, X.W., 2011b. Effect of anaerobic reaction time on denitrifying phosphorus removal and N2O production. Bioresource Technology, 102(10), 5674–5684. https://doi.org/10.1016/j.biortech.2011.02.080.

Wang, Y.Y., Geng, J.J., Guo, G., Wang, C., Liu, S.H., 2011c. N2O production in anaerobic/anoxic denitrifying phosphorus removal process: The effects of carbon sources shock. Chemical Engineering Journal, 172(2–3), 999–1007. https://doi.org/10.1016/j.cej.2011.07.014.

Wang, Y.Y., Zhou, S., Liu, Y., Wang, H., Stephenson, T., Jiang, X.X., 2014. Nitrite survival and nitrous oxide production of denitrifying phosphorus removal sludges in long-term nitrite/nitrate-fed sequencing batch reactors. Water Research, 67, 33–45. https://doi.org/10.1016/j.watres.2014.08.052.

Wu, C.Y., 2010. A2/O Denitrifying Phosphorus Process and Its Optimal Control. Ph. D. Dissertation. Harbin University of Technology, Harbin (in Chinese).

Yang, Y.Y., Zeng, W., Liu, J.R., Li, L., Wang, X.D., 2010. Effect of nitrite on biological phosphorus removal in wastewater. Microbiology China, 37(4), 586–593. https://doi.org/10.13344/j.microbiol.china.2010.04.013 (in Chinese).

Zafiriadis, I., Ntougias, S., Nikolaidis, C., Kapagiannidis, A.G., Aivasidis, A., 2011. Denitrifying polyphosphate accumulating organisms population and nitrite reductase gene diversity shift in a DEPHANOX-type activated sludge system fed with municipal wastewater. Journal of Bioscience and Bioengineering, 111(2), 185–192. https://doi.org/10.1016/j.jbiosc.2010.09.016.

Zeng, W., Li, L., Yang, Y..Y., Wang, X.D., Peng, Y.Z., 2011. Denitrifying phosphorus removal and impact of nitrite accumulation on phosphorus removal in a continuous anaerobic-anoxic-aerobic (A2O) process treating domestic wastewater. Enzyme and Microbial Technology, 48(2), 134–142. https://doi.org/10.1016/j.enzmictec.2010.10.010.

Zhou, K.Q., Liu, H., Sun, Y.F., Zhou, Y.P., Liu, J.P., 2007. Screening and enrichment of denitrifying phosphorus accumulating bacteria using nitrite as electronic acceptor. Environmental Engineering, 1(8), 126–131. https://doi.org/10.3969/j.issn.1673-9108.2007.08.027.

Zhou, S.Q., Zhang, X.J., Feng, L.Y., 2010. Effect of different types of electron acceptors on the anoxic phosphorus uptake activity of denitrifying phosphorus removing bacteria. Bioresource Technology, 101(6), 1603–1610. https://doi.org/10.1016/j.biortech.2009.09.032.

 

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